Increased CO2 and Ocean Acidification

CO2 Manipulation ExperimentCARBON DIOXIDE  MANIPULATION EXPERIMENTS IN THE LABORATORY

Thalassiosira weissflogii (a diatom species) was grown under three different CO2 environments: 150 ppm (pre-industrial concentrations), 400 ppm (present day), and 800 ppm (end of the century prediction).

  • Phytoplankton biomass and growth rates were monitored over time by taking samples for in vivo chlorophyll a measurements and cell counts using FlowCAM®
  • Changes in absorption spectra of pigments were examined using a spectrophotometer
  • Changes in the photosynthetic capacity of the cells were examined using a Fast Repetition Rate Fluorometer (FRRF)

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